Selective staining of alpha1-antitrypsin (alpha1-protease inhibitor) with Schiff's reagent after separation from serum by analytical isoelectrofocusing in polyacrylamide gel.
نویسندگان
چکیده
After electrofocusing serum from human and various animals in the pH range 4.0-6.0, only the isoproteins of antitrypsin stain with Schiff's reagent. As an additional advantage, this staining provides information about the relative contents of the Schiff-positive carbohydrates of various antitrypsins.
منابع مشابه
Alpha-1-antitrypsin deficiency in children: liver disease is not reflected by low serum levels of alpha-1-antitrypsin - a study on 48 pediatric patients.
BACKGROUND Alpha-1-antitrypsin (alpha1-AT) is an important protease inhibitor. The phenotypes are characterized by a low total serum alpha1-AT or by an abnormal protein accumulating in the hepatocytes. The aim of our study was to examine a correlation of total serum alpha1-AT, phenotype, and liver involvement in pediatric patients. METHODS 48 patients, deficient for alpha1-AT were included. T...
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عنوان ژورنال:
- Clinical chemistry
دوره 24 1 شماره
صفحات -
تاریخ انتشار 1978